Novel Thiadiazole Derivative as a Schiff Base and Its Metal Complexes: Synthesis, Structural Characterization and Cytotoxic Activity

Abstract

This study presents the synthesis and characterization of a Schiff base ligand, [(E)-4-(((5-ethyl-1,3,4-thiadiazol-2- yl)imino)methyl)benzene-1,2,3-triol] (ETIMBT), achieved by condensing equimolar amounts of 2-amino-5-ethyl-1,3,4-thiadiazole and 2,3,4-trihydroxybenzaldehyde. The ligand was subsequently reacted with Ag(I), Ni(II), and Cu(II) in the ratios of 1:1, 2:1, and 2:1, respectively, to form the corresponding metal complexes. These complexes were characterized using CHN analysis, FT-IR, electronic spectra, �H NMR spectra, and mass spectrometry. The in vitro anticancer activity of the ligand and its metal complexes was evaluated against HeLa and PC3 cell lines. Notably, the ETIMBT-Cu(II) complex demonstrated significant cytotoxic activity against HeLa cells, highlighting its potential as an anticancer agent.